Bromine in PDB 1fz8: Methane Monooxygenase Hydroxylase, Form II Cocrystallized with Dibromomethane

All present enzymatic activity of Methane Monooxygenase Hydroxylase, Form II Cocrystallized with Dibromomethane:
1.14.13.25;

The structure of Methane Monooxygenase Hydroxylase, Form II Cocrystallized with Dibromomethane, PDB code: 1fz8 was solved by D.A.Whittington, A.C.Rosenzweig, C.A.Frederick, S.J.Lippard, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	23.50 / 2.10
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	71.170, 171.990, 221.330, 90.00, 90.00, 90.00
R / Rfree (%)	20.1 / 25

The structure of Methane Monooxygenase Hydroxylase, Form II Cocrystallized with Dibromomethane also contains other interesting chemical elements:

Iron	(Fe)	4 atoms
Calcium	(Ca)	3 atoms

Pages:

>>> Page 1 <<< Page 2, Binding sites: 11 - 20;

Binding sites:

The binding sites of Bromine atom in the Methane Monooxygenase Hydroxylase, Form II Cocrystallized with Dibromomethane (pdb code 1fz8). This binding sites where shown within 5.0 Angstroms radius around Bromine atom.
In total 20 binding sites of Bromine where determined in the Methane Monooxygenase Hydroxylase, Form II Cocrystallized with Dibromomethane, PDB code: 1fz8:
Jump to Bromine binding site number: 1; 2; 3; 4; 5; 6; 7; 8; 9; 10;

Bromine binding site 1 out of 20 in the Methane Monooxygenase Hydroxylase, Form II Cocrystallized with Dibromomethane


Mono view


Stereo pair view

A full contact list of Bromine with other atoms in the Br binding site number 1 of Methane Monooxygenase Hydroxylase, Form II Cocrystallized with Dibromomethane within 5.0Å range: probe atom residue distance (Å) B Occ A:Br9006 b:29.4 occ:1.00 BR1 A:2BM9006 0.0 29.4 1.0 C A:2BM9006 1.9 29.3 1.0 BR2 A:2BM9006 3.3 30.4 1.0 CA A:GLY293 3.6 22.7 1.0 N A:GLY293 3.7 20.7 1.0 CD2 A:LEU361 3.8 25.1 1.0 CE1 A:TYR347 3.9 25.2 1.0 C A:TYR292 3.9 20.7 1.0 O A:MET288 4.0 26.1 1.0 O A:TYR292 4.1 21.9 1.0 CD2 A:LEU289 4.1 18.7 1.0 CB A:TYR292 4.3 19.6 1.0 CA A:LEU289 4.3 23.1 1.0 O A:HOH9047 4.4 19.3 1.0 OG1 A:THR102 4.4 16.4 1.0 CD1 A:TYR347 4.5 20.4 1.0 O A:HOH9048 4.7 18.0 1.0 C A:MET288 4.7 25.3 1.0 CA A:TYR292 4.7 19.9 1.0 O A:LEU289 4.7 21.1 1.0 CG A:MET288 4.8 26.1 1.0 N A:LEU289 4.8 24.7 1.0 CZ A:TYR347 4.9 20.4 1.0 CE A:MET288 4.9 28.9 1.0 OH A:TYR347 5.0 22.0 1.0

Bromine binding site 2 out of 20 in the Methane Monooxygenase Hydroxylase, Form II Cocrystallized with Dibromomethane


Mono view


Stereo pair view

A full contact list of Bromine with other atoms in the Br binding site number 2 of Methane Monooxygenase Hydroxylase, Form II Cocrystallized with Dibromomethane within 5.0Å range: probe atom residue distance (Å) B Occ A:Br9006 b:30.4 occ:1.00 BR2 A:2BM9006 0.0 30.4 1.0 C A:2BM9006 1.9 29.3 1.0 BR1 A:2BM9006 3.3 29.4 1.0 CD1 A:PHE359 3.7 12.5 1.0 CA A:THR102 3.8 18.4 1.0 CD2 A:LEU361 3.8 25.1 1.0 CG2 A:VAL105 3.8 17.7 1.0 CD2 A:LEU289 3.9 18.7 1.0 CG A:PHE359 4.0 16.1 1.0 N A:THR102 4.2 18.3 1.0 OG1 A:THR102 4.2 16.4 1.0 O A:GLU101 4.2 18.6 1.0 CE1 A:PHE359 4.2 13.4 1.0 C A:GLU101 4.3 15.9 1.0 CB A:PHE359 4.4 18.3 1.0 O A:HOH9048 4.5 18.0 1.0 CB A:VAL105 4.5 18.0 1.0 CB A:THR102 4.6 14.7 1.0 CD2 A:PHE359 4.7 12.1 1.0 C A:THR102 4.8 18.3 1.0 O A:THR102 4.8 20.3 1.0 CZ A:PHE359 4.9 16.2 1.0 CB A:GLU101 5.0 16.8 1.0

Bromine binding site 3 out of 20 in the Methane Monooxygenase Hydroxylase, Form II Cocrystallized with Dibromomethane


Mono view


Stereo pair view

A full contact list of Bromine with other atoms in the Br binding site number 3 of Methane Monooxygenase Hydroxylase, Form II Cocrystallized with Dibromomethane within 5.0Å range: probe atom residue distance (Å) B Occ A:Br9007 b:35.2 occ:0.79 BR1 A:2BM9007 0.0 35.2 0.8 C A:2BM9007 1.9 33.7 0.8 BR2 A:2BM9007 3.1 30.4 0.8 CD2 A:LEU216 3.5 29.8 1.0 CE2 A:PHE188 4.1 37.0 1.0 CB A:LEU110 4.2 18.3 1.0 CD2 A:LEU286 4.3 27.7 1.0 O A:VAL106 4.4 17.4 1.0 CZ A:PHE188 4.5 35.0 1.0 BR2 A:2BM9008 4.5 29.2 0.6 CA A:LEU110 4.6 18.4 1.0 CG A:LEU216 4.6 32.0 1.0 CD1 A:LEU110 4.6 20.1 1.0 CE A:MET184 4.7 32.0 1.0 CG1 A:VAL106 4.7 20.2 1.0 N A:LEU110 4.7 17.2 1.0 CE1 A:PHE282 4.8 33.8 1.0 CD1 A:LEU216 4.9 34.5 1.0 CG A:LEU110 4.9 22.8 1.0

Bromine binding site 4 out of 20 in the Methane Monooxygenase Hydroxylase, Form II Cocrystallized with Dibromomethane


Mono view


Stereo pair view

A full contact list of Bromine with other atoms in the Br binding site number 4 of Methane Monooxygenase Hydroxylase, Form II Cocrystallized with Dibromomethane within 5.0Å range: probe atom residue distance (Å) B Occ A:Br9007 b:30.4 occ:0.79 BR2 A:2BM9007 0.0 30.4 0.8 C A:2BM9007 1.9 33.7 0.8 BR1 A:2BM9007 3.1 35.2 0.8 CE A:MET184 3.3 32.0 1.0 CD1 A:LEU289 3.3 18.7 1.0 CB A:PHE109 4.1 18.6 1.0 CA A:VAL106 4.1 18.9 1.0 BR2 A:2BM9008 4.3 29.2 0.6 CD2 A:PHE109 4.3 12.5 1.0 O A:VAL105 4.5 19.5 1.0 CG1 A:VAL105 4.5 13.6 1.0 CD2 A:LEU286 4.5 27.7 1.0 CG1 A:VAL285 4.6 21.1 1.0 CG2 A:VAL106 4.6 22.3 1.0 O A:VAL106 4.6 17.4 1.0 CG A:PHE109 4.7 17.3 1.0 N A:VAL106 4.7 17.5 1.0 SD A:MET184 4.8 30.8 1.0 C A:VAL105 4.8 19.3 1.0 CB A:VAL106 4.8 19.8 1.0 CG A:LEU289 4.8 21.2 1.0 C A:VAL106 4.9 17.7 1.0

Bromine binding site 5 out of 20 in the Methane Monooxygenase Hydroxylase, Form II Cocrystallized with Dibromomethane


Mono view


Stereo pair view

A full contact list of Bromine with other atoms in the Br binding site number 5 of Methane Monooxygenase Hydroxylase, Form II Cocrystallized with Dibromomethane within 5.0Å range: probe atom residue distance (Å) B Occ A:Br9008 b:28.2 occ:0.58 BR1 A:2BM9008 0.0 28.2 0.6 C A:2BM9008 1.9 30.1 0.6 BR2 A:2BM9008 3.2 29.2 0.6 CD2 A:PHE290 3.2 27.4 1.0 CE2 A:PHE290 3.4 29.3 1.0 CH2 A:TRP99 3.5 23.4 1.0 CB A:LEU289 3.7 20.7 1.0 N A:PHE290 3.8 23.1 1.0 C A:LEU289 3.8 22.2 1.0 CZ3 A:TRP99 3.9 23.6 1.0 CG2 A:VAL106 3.9 22.3 1.0 CA A:PHE290 4.0 23.3 1.0 O A:LEU289 4.0 21.1 1.0 CG A:PHE290 4.0 25.3 1.0 CG2 A:THR102 4.0 13.1 1.0 CZ A:PHE290 4.3 28.3 1.0 CA A:LEU289 4.4 23.1 1.0 CZ2 A:TRP99 4.5 22.7 1.0 CB A:PHE290 4.6 24.4 1.0 CG2 A:VAL220 4.6 23.4 1.0 CD1 A:PHE290 4.8 26.4 1.0 O A:LEU286 4.9 27.5 1.0 CG A:LEU289 4.9 21.2 1.0 CE1 A:PHE290 4.9 26.9 1.0 CD1 A:LEU289 5.0 18.7 1.0

Bromine binding site 6 out of 20 in the Methane Monooxygenase Hydroxylase, Form II Cocrystallized with Dibromomethane


Mono view


Stereo pair view

A full contact list of Bromine with other atoms in the Br binding site number 6 of Methane Monooxygenase Hydroxylase, Form II Cocrystallized with Dibromomethane within 5.0Å range: probe atom residue distance (Å) B Occ A:Br9008 b:29.2 occ:0.58 BR2 A:2BM9008 0.0 29.2 0.6 C A:2BM9008 1.9 30.1 0.6 BR1 A:2BM9008 3.2 28.2 0.6 CD1 A:LEU289 3.7 18.7 1.0 CD2 A:LEU286 3.7 27.7 1.0 CD1 A:LEU216 3.7 34.5 1.0 CG2 A:VAL106 3.8 22.3 1.0 CB A:LEU289 4.0 20.7 1.0 CA A:LEU286 4.3 28.4 1.0 BR2 A:2BM9007 4.3 30.4 0.8 CG2 A:VAL220 4.4 23.4 1.0 CG1 A:VAL106 4.4 20.2 1.0 CG A:LEU289 4.5 21.2 1.0 O A:LEU286 4.5 27.5 1.0 BR1 A:2BM9007 4.5 35.2 0.8 CZ A:PHE290 4.6 28.3 1.0 CB A:LEU286 4.6 28.7 1.0 CE2 A:PHE290 4.6 29.3 1.0 CB A:VAL106 4.7 19.8 1.0 CG A:LEU286 4.8 30.2 1.0 CE1 A:PHE290 4.8 26.9 1.0 CG A:LEU216 4.9 32.0 1.0 CD2 A:PHE290 4.9 27.4 1.0 C A:LEU286 4.9 27.5 1.0 CD2 A:LEU216 5.0 29.8 1.0

Bromine binding site 7 out of 20 in the Methane Monooxygenase Hydroxylase, Form II Cocrystallized with Dibromomethane


Mono view


Stereo pair view

A full contact list of Bromine with other atoms in the Br binding site number 7 of Methane Monooxygenase Hydroxylase, Form II Cocrystallized with Dibromomethane within 5.0Å range: probe atom residue distance (Å) B Occ B:Br9001 b:34.3 occ:0.79 BR1 B:2BM9001 0.0 34.3 0.8 C B:2BM9001 1.9 25.8 0.8 BR2 B:2BM9001 3.1 29.5 0.8 CD2 B:LEU216 3.6 36.9 1.0 CB B:LEU110 4.0 23.7 1.0 CE2 B:PHE188 4.1 34.6 1.0 CD2 B:LEU286 4.3 23.5 1.0 O B:VAL106 4.3 18.6 1.0 CA B:LEU110 4.4 22.1 1.0 CD1 B:LEU110 4.5 21.2 1.0 N B:LEU110 4.5 18.7 1.0 CZ B:PHE188 4.6 32.5 1.0 CG B:LEU110 4.7 20.9 1.0 BR2 B:2BM9002 4.7 33.0 0.6 CG B:LEU216 4.8 35.1 1.0 CE1 B:PHE282 4.8 29.3 1.0 CG1 B:VAL106 4.9 17.1 1.0 CD2 B:LEU110 5.0 26.7 1.0

Bromine binding site 8 out of 20 in the Methane Monooxygenase Hydroxylase, Form II Cocrystallized with Dibromomethane


Mono view


Stereo pair view

A full contact list of Bromine with other atoms in the Br binding site number 8 of Methane Monooxygenase Hydroxylase, Form II Cocrystallized with Dibromomethane within 5.0Å range: probe atom residue distance (Å) B Occ B:Br9001 b:29.5 occ:0.79 BR2 B:2BM9001 0.0 29.5 0.8 C B:2BM9001 1.9 25.8 0.8 BR1 B:2BM9001 3.1 34.3 0.8 CD1 B:LEU289 3.4 19.3 1.0 CB B:PHE109 3.9 16.2 1.0 CA B:VAL106 4.1 18.8 1.0 O B:VAL105 4.3 20.7 1.0 CG1 B:VAL105 4.4 19.2 1.0 SD B:MET184 4.4 31.2 1.0 CD2 B:PHE109 4.4 13.2 1.0 BR2 B:2BM9002 4.4 33.0 0.6 CD2 B:LEU286 4.4 23.5 1.0 CG B:PHE109 4.6 15.4 1.0 O B:VAL106 4.6 18.6 1.0 CG1 B:VAL285 4.6 11.1 1.0 N B:VAL106 4.6 15.8 1.0 C B:VAL105 4.7 18.3 1.0 CG2 B:VAL106 4.8 18.8 1.0 C B:VAL106 4.9 20.0 1.0 CG B:LEU289 4.9 22.2 1.0 CB B:VAL106 5.0 16.6 1.0

Bromine binding site 9 out of 20 in the Methane Monooxygenase Hydroxylase, Form II Cocrystallized with Dibromomethane


Mono view


Stereo pair view

A full contact list of Bromine with other atoms in the Br binding site number 9 of Methane Monooxygenase Hydroxylase, Form II Cocrystallized with Dibromomethane within 5.0Å range: probe atom residue distance (Å) B Occ B:Br9002 b:27.2 occ:0.64 BR1 B:2BM9002 0.0 27.2 0.6 C B:2BM9002 1.9 29.9 0.6 CD2 B:PHE290 3.2 26.8 1.0 BR2 B:2BM9002 3.2 33.0 0.6 CE2 B:PHE290 3.4 25.4 1.0 CH2 B:TRP99 3.5 18.5 1.0 N B:PHE290 3.7 17.8 1.0 C B:LEU289 3.8 18.0 1.0 CB B:LEU289 3.8 20.2 1.0 CA B:PHE290 3.9 19.1 1.0 O B:LEU289 3.9 20.9 1.0 CZ3 B:TRP99 3.9 18.2 1.0 CG B:PHE290 4.0 22.4 1.0 CG2 B:VAL106 4.0 18.8 1.0 CG2 B:THR102 4.1 15.9 1.0 CZ B:PHE290 4.4 21.9 1.0 CA B:LEU289 4.5 19.5 1.0 CB B:PHE290 4.5 21.0 1.0 CZ2 B:TRP99 4.5 21.6 1.0 CG2 B:VAL220 4.6 21.7 1.0 CD1 B:PHE290 4.8 23.6 1.0 O B:LEU286 5.0 18.6 1.0 CE1 B:PHE290 5.0 23.9 1.0

Bromine binding site 10 out of 20 in the Methane Monooxygenase Hydroxylase, Form II Cocrystallized with Dibromomethane


Mono view


Stereo pair view

A full contact list of Bromine with other atoms in the Br binding site number 10 of Methane Monooxygenase Hydroxylase, Form II Cocrystallized with Dibromomethane within 5.0Å range: probe atom residue distance (Å) B Occ B:Br9002 b:33.0 occ:0.64 BR2 B:2BM9002 0.0 33.0 0.6 C B:2BM9002 1.9 29.9 0.6 BR1 B:2BM9002 3.2 27.2 0.6 CD2 B:LEU286 3.3 23.5 1.0 CD1 B:LEU289 3.7 19.3 1.0 CD1 B:LEU216 3.8 38.6 1.0 CG2 B:VAL106 3.9 18.8 1.0 CB B:LEU289 4.0 20.2 1.0 CG2 B:VAL220 4.3 21.7 1.0 CA B:LEU286 4.4 17.0 1.0 BR2 B:2BM9001 4.4 29.5 0.8 CE2 B:PHE290 4.5 25.4 1.0 CZ B:PHE290 4.5 21.9 1.0 CG B:LEU289 4.5 22.2 1.0 O B:LEU286 4.5 18.6 1.0 CG B:LEU286 4.6 21.3 1.0 CB B:LEU286 4.6 18.7 1.0 CG1 B:VAL106 4.6 17.1 1.0 BR1 B:2BM9001 4.7 34.3 0.8 CG B:LEU216 4.8 35.1 1.0 CD2 B:LEU216 4.8 36.9 1.0 CD2 B:PHE290 4.8 26.8 1.0 CE1 B:PHE290 4.8 23.9 1.0 CB B:VAL106 4.8 16.6 1.0 C B:LEU286 5.0 16.6 1.0

D.A.Whittington, A.C.Rosenzweig, C.A.Frederick, S.J.Lippard. Xenon and Halogenated Alkanes Track Putative Substrate Binding Cavities in the Soluble Methane Monooxygenase Hydroxylase. Biochemistry V. 40 3476 2001.
ISSN: ISSN 0006-2960
PubMed: 11297413
DOI: 10.1021/BI0022487