Bromine in PDB 3ejy: Structure of E203H Mutant of E.Coli Cl-/H+ Antiporter, Clc-EC1

The structure of Structure of E203H Mutant of E.Coli Cl-/H+ Antiporter, Clc-EC1, PDB code: 3ejy was solved by H.-H.Lim, C.Miller, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	58.52 / 3.20
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	231.516, 96.830, 172.790, 90.00, 132.81, 90.00
R / Rfree (%)	24.4 / 27.9

The binding sites of Bromine atom in the Structure of E203H Mutant of E.Coli Cl-/H+ Antiporter, Clc-EC1 (pdb code 3ejy). This binding sites where shown within 5.0 Angstroms radius around Bromine atom.
In total 4 binding sites of Bromine where determined in the Structure of E203H Mutant of E.Coli Cl-/H+ Antiporter, Clc-EC1, PDB code: 3ejy:
Jump to Bromine binding site number: 1; 2; 3; 4;

Bromine binding site 1 out of 4 in the Structure of E203H Mutant of E.Coli Cl-/H+ Antiporter, Clc-EC1


Mono view


Stereo pair view

A full contact list of Bromine with other atoms in the Br binding site number 1 of Structure of E203H Mutant of E.Coli Cl-/H+ Antiporter, Clc-EC1 within 5.0Å range: probe atom residue distance (Å) B Occ A:Br501 b:0.0 occ:1.00 OG A:SER107 1.7 91.1 1.0 OH A:TYR445 1.8 96.5 1.0 CB A:SER107 3.1 90.6 1.0 CZ A:TYR445 3.1 97.1 1.0 N A:ILE356 3.2 83.9 1.0 CA A:GLY355 3.2 83.9 1.0 CD2 A:PHE357 3.7 85.9 1.0 CD1 A:ILE109 3.7 89.8 1.0 C A:GLY355 3.7 83.9 1.0 N A:PHE357 3.9 84.3 1.0 CE1 A:TYR445 3.9 96.9 1.0 CE2 A:TYR445 4.1 97.0 1.0 N A:GLY149 4.1 86.6 1.0 OE1 A:GLU148 4.2 89.5 1.0 CA A:SER107 4.2 90.3 1.0 CG1 A:ILE109 4.3 89.7 1.0 CA A:ILE356 4.3 83.8 1.0 CA A:GLY149 4.4 85.3 1.0 CB A:PHE357 4.4 84.5 1.0 CG A:PHE357 4.4 84.9 1.0 CB A:GLU148 4.4 87.9 1.0 CE2 A:PHE357 4.5 86.5 1.0 N A:GLY355 4.5 83.9 1.0 CG2 A:ILE356 4.6 83.2 1.0 C A:ILE356 4.6 84.1 1.0 CA A:PHE357 4.7 84.4 1.0 CB A:ILE356 4.8 83.5 1.0 O A:GLY355 4.9 83.8 1.0

Bromine binding site 2 out of 4 in the Structure of E203H Mutant of E.Coli Cl-/H+ Antiporter, Clc-EC1


Mono view


Stereo pair view

A full contact list of Bromine with other atoms in the Br binding site number 2 of Structure of E203H Mutant of E.Coli Cl-/H+ Antiporter, Clc-EC1 within 5.0Å range: probe atom residue distance (Å) B Occ A:Br502 b:0.0 occ:1.00 N A:SER107 3.2 90.2 1.0 CZ A:PHE348 3.5 86.5 1.0 CA A:GLY106 3.5 90.6 1.0 C A:GLY106 3.8 90.3 1.0 CE1 A:PHE348 4.1 86.1 1.0 CG2 A:ILE448 4.1 95.7 1.0 N A:GLY108 4.1 90.2 1.0 CG A:PRO110 4.2 90.0 1.0 CA A:SER107 4.2 90.3 1.0 CB A:SER107 4.3 90.6 1.0 CD A:PRO110 4.3 89.9 1.0 CE2 A:PHE348 4.3 85.4 1.0 CD1 A:ILE448 4.3 95.4 1.0 N A:GLY106 4.6 91.4 1.0 O A:GLY105 4.6 92.2 1.0 C A:SER107 4.7 90.1 1.0 O A:GLY106 5.0 89.9 1.0

Bromine binding site 3 out of 4 in the Structure of E203H Mutant of E.Coli Cl-/H+ Antiporter, Clc-EC1


Mono view


Stereo pair view

A full contact list of Bromine with other atoms in the Br binding site number 3 of Structure of E203H Mutant of E.Coli Cl-/H+ Antiporter, Clc-EC1 within 5.0Å range: probe atom residue distance (Å) B Occ B:Br503 b:0.0 occ:1.00 OH B:TYR445 1.9 96.5 1.0 OG B:SER107 2.2 89.4 1.0 N B:ILE356 2.9 83.0 1.0 CA B:GLY355 3.1 82.2 1.0 CZ B:TYR445 3.2 97.1 1.0 CB B:SER107 3.3 87.4 1.0 CD2 B:PHE357 3.4 87.0 1.0 C B:GLY355 3.5 82.5 1.0 CD1 B:ILE109 3.5 86.5 1.0 N B:PHE357 3.6 84.7 1.0 CE2 B:TYR445 3.9 97.1 1.0 OE1 B:GLU148 4.0 89.6 1.0 CE2 B:PHE357 4.0 87.4 1.0 CA B:ILE356 4.0 83.3 1.0 CG B:PHE357 4.2 86.0 1.0 CE1 B:TYR445 4.2 96.4 1.0 CG2 B:ILE356 4.2 82.8 1.0 C B:ILE356 4.3 84.0 1.0 N B:GLY149 4.3 86.3 1.0 CB B:PHE357 4.3 85.4 1.0 CA B:SER107 4.4 86.9 1.0 N B:GLY355 4.4 81.9 1.0 CA B:GLY149 4.6 84.8 1.0 CA B:PHE357 4.6 85.1 1.0 CB B:ILE356 4.6 83.2 1.0 CB B:GLU148 4.7 87.4 1.0 CG1 B:ILE109 4.7 86.1 1.0 O B:GLY355 4.7 82.4 1.0 CD B:GLU148 5.0 90.3 1.0

Bromine binding site 4 out of 4 in the Structure of E203H Mutant of E.Coli Cl-/H+ Antiporter, Clc-EC1


Mono view


Stereo pair view

A full contact list of Bromine with other atoms in the Br binding site number 4 of Structure of E203H Mutant of E.Coli Cl-/H+ Antiporter, Clc-EC1 within 5.0Å range: probe atom residue distance (Å) B Occ B:Br504 b:0.0 occ:1.00 N B:SER107 3.2 86.6 1.0 CZ B:PHE348 3.7 86.1 1.0 CA B:GLY106 3.7 86.9 1.0 N B:GLY108 3.8 86.5 1.0 CG B:PRO110 3.8 86.3 1.0 C B:GLY106 3.9 86.6 1.0 CD B:PRO110 3.9 86.3 1.0 CA B:SER107 4.1 86.9 1.0 CB B:SER107 4.1 87.4 1.0 CG2 B:ILE448 4.1 95.9 1.0 CE1 B:PHE348 4.2 85.7 1.0 C B:SER107 4.4 86.5 1.0 O B:GLY105 4.5 88.1 1.0 CE2 B:PHE348 4.6 85.0 1.0 CD1 B:ILE448 4.7 95.6 1.0 CA B:GLY108 4.8 86.3 1.0 N B:GLY106 4.8 87.6 1.0 CB B:PRO110 4.9 86.2 1.0

H.H.Lim, C.Miller. Intracellular Proton-Transfer Mutants in A Clc Cl-/H+ Exchanger. J.Gen.Physiol. V. 133 131 2009.
ISSN: ISSN 0022-1295
PubMed: 19139174
DOI: 10.1085/JGP.200810112