Bromine in PDB 3ejz: Structure of E203V Mutant E.Coli Cl-/H+ Exchanger, Clc-EC1

The structure of Structure of E203V Mutant E.Coli Cl-/H+ Exchanger, Clc-EC1, PDB code: 3ejz was solved by H.-H.Lim, C.Miller, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	58.76 / 2.90
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	231.259, 96.415, 170.150, 90.00, 131.78, 90.00
R / Rfree (%)	25.2 / 28.1

The binding sites of Bromine atom in the Structure of E203V Mutant E.Coli Cl-/H+ Exchanger, Clc-EC1 (pdb code 3ejz). This binding sites where shown within 5.0 Angstroms radius around Bromine atom.
In total 4 binding sites of Bromine where determined in the Structure of E203V Mutant E.Coli Cl-/H+ Exchanger, Clc-EC1, PDB code: 3ejz:
Jump to Bromine binding site number: 1; 2; 3; 4;

Bromine binding site 1 out of 4 in the Structure of E203V Mutant E.Coli Cl-/H+ Exchanger, Clc-EC1


Mono view


Stereo pair view

A full contact list of Bromine with other atoms in the Br binding site number 1 of Structure of E203V Mutant E.Coli Cl-/H+ Exchanger, Clc-EC1 within 5.0Å range: probe atom residue distance (Å) B Occ A:Br474 b:0.0 occ:1.00 OH A:TYR445 1.7 0.7 1.0 CZ A:TYR445 3.0 0.2 1.0 N A:ILE356 3.2 82.3 1.0 CA A:GLY355 3.2 0.3 1.0 OG A:SER107 3.5 0.2 1.0 C A:GLY355 3.7 100.0 1.0 CE2 A:TYR445 3.7 0.5 1.0 CB A:SER107 3.7 0.5 1.0 N A:PHE357 3.8 88.0 1.0 OE1 A:GLU148 3.9 88.6 1.0 N A:GLY149 4.0 79.0 1.0 CE1 A:TYR445 4.1 0.7 1.0 CB A:PHE357 4.1 88.0 1.0 CD1 A:ILE109 4.1 83.4 1.0 CA A:GLY149 4.2 78.2 1.0 CA A:ILE356 4.3 82.4 1.0 CG1 A:ILE109 4.4 83.1 1.0 CG2 A:ILE356 4.4 82.4 1.0 CA A:SER107 4.4 0.4 1.0 N A:GLY355 4.4 0.4 1.0 C A:ILE356 4.5 82.5 1.0 CB A:GLU148 4.5 85.5 1.0 CA A:PHE357 4.5 87.9 1.0 CD1 A:PHE357 4.6 91.6 1.0 C A:GLU148 4.8 85.5 1.0 CB A:ILE356 4.8 82.3 1.0 CD A:GLU148 4.8 89.9 1.0 CG A:PHE357 4.8 89.8 1.0 O A:GLY355 4.9 0.2 1.0

Bromine binding site 2 out of 4 in the Structure of E203V Mutant E.Coli Cl-/H+ Exchanger, Clc-EC1


Mono view


Stereo pair view

A full contact list of Bromine with other atoms in the Br binding site number 2 of Structure of E203V Mutant E.Coli Cl-/H+ Exchanger, Clc-EC1 within 5.0Å range: probe atom residue distance (Å) B Occ A:Br475 b:0.0 occ:1.00 N A:SER107 2.9 0.6 1.0 CB A:SER107 3.6 0.5 1.0 CA A:GLY106 3.7 97.5 1.0 C A:GLY106 3.8 96.9 1.0 CA A:SER107 3.8 0.4 1.0 CZ A:PHE348 3.8 83.8 1.0 CG2 A:ILE448 4.1 91.9 1.0 N A:GLY108 4.1 0.3 1.0 CG A:PRO110 4.1 96.3 1.0 CE1 A:PHE348 4.3 83.4 1.0 CD A:PRO110 4.4 96.5 1.0 CD1 A:ILE448 4.4 92.2 1.0 C A:SER107 4.5 0.2 1.0 OG A:SER107 4.6 0.2 1.0 O A:GLY105 4.7 0.5 1.0 CE2 A:PHE348 4.7 83.9 1.0 N A:GLY106 4.9 98.7 1.0 O A:GLY106 4.9 96.4 1.0

Bromine binding site 3 out of 4 in the Structure of E203V Mutant E.Coli Cl-/H+ Exchanger, Clc-EC1


Mono view


Stereo pair view

A full contact list of Bromine with other atoms in the Br binding site number 3 of Structure of E203V Mutant E.Coli Cl-/H+ Exchanger, Clc-EC1 within 5.0Å range: probe atom residue distance (Å) B Occ B:Br474 b:0.0 occ:1.00 OH B:TYR445 1.4 0.6 1.0 CZ B:TYR445 2.8 0.6 1.0 N B:ILE356 3.2 73.6 1.0 CA B:GLY355 3.4 82.0 1.0 OG B:SER107 3.5 0.8 1.0 CE2 B:TYR445 3.5 0.5 1.0 N B:PHE357 3.7 95.4 1.0 CB B:SER107 3.7 0.7 1.0 C B:GLY355 3.8 81.4 1.0 CE1 B:TYR445 3.8 0.7 1.0 OE1 B:GLU148 4.0 89.9 1.0 CB B:PHE357 4.0 95.5 1.0 CG2 B:ILE356 4.2 72.3 1.0 CD1 B:ILE109 4.2 91.1 1.0 CA B:ILE356 4.2 73.4 1.0 N B:GLY149 4.3 98.1 1.0 C B:ILE356 4.4 74.0 1.0 CA B:PHE357 4.4 95.2 1.0 CD1 B:PHE357 4.4 99.2 1.0 CG1 B:ILE109 4.4 90.0 1.0 CA B:GLY149 4.5 96.9 1.0 CA B:SER107 4.5 0.5 1.0 N B:GLY355 4.7 82.8 1.0 CB B:ILE356 4.7 73.0 1.0 CB B:GLU148 4.7 89.8 1.0 CG B:PHE357 4.7 97.3 1.0 CD2 B:TYR445 4.9 0.0 1.0 CD B:GLU148 4.9 91.0 1.0

Bromine binding site 4 out of 4 in the Structure of E203V Mutant E.Coli Cl-/H+ Exchanger, Clc-EC1


Mono view


Stereo pair view

A full contact list of Bromine with other atoms in the Br binding site number 4 of Structure of E203V Mutant E.Coli Cl-/H+ Exchanger, Clc-EC1 within 5.0Å range: probe atom residue distance (Å) B Occ B:Br475 b:0.0 occ:1.00 N B:SER107 3.0 0.7 1.0 CB B:SER107 3.6 0.7 1.0 CA B:SER107 3.8 0.5 1.0 CZ B:PHE348 3.9 78.8 1.0 C B:GLY106 3.9 92.8 1.0 CG B:PRO110 3.9 88.9 1.0 CG2 B:ILE448 3.9 95.4 1.0 CA B:GLY106 3.9 93.4 1.0 N B:GLY108 4.0 86.4 1.0 CD B:PRO110 4.2 89.0 1.0 CD1 B:ILE448 4.3 95.0 1.0 CE1 B:PHE348 4.3 78.5 1.0 C B:SER107 4.5 0.1 1.0 OG B:SER107 4.5 0.8 1.0 CE2 B:PHE348 4.7 78.2 1.0 O B:GLY105 4.8 91.3 1.0 CB B:ILE448 5.0 95.7 1.0

H.H.Lim, C.Miller. Intracellular Proton-Transfer Mutants in A Clc Cl-/H+ Exchanger. J.Gen.Physiol. V. 133 131 2009.
ISSN: ISSN 0022-1295
PubMed: 19139174
DOI: 10.1085/JGP.200810112