Bromine in PDB 3h40: Binary Complex of Human Dna Polymerase Iota with Template U/T

All present enzymatic activity of Binary Complex of Human Dna Polymerase Iota with Template U/T:
2.7.7.7;

The structure of Binary Complex of Human Dna Polymerase Iota with Template U/T, PDB code: 3h40 was solved by R.Jain, D.T.Nair, R.E.Johnson, L.Prakash, S.Prakash, A.K.Aggarwal, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	43.67 / 2.30
Space group	P 65 2 2
Cell size a, b, c (Å), α, β, γ (°)	98.243, 98.243, 203.528, 90.00, 90.00, 120.00
R / Rfree (%)	25.3 / 27.7

The binding sites of Bromine atom in the Binary Complex of Human Dna Polymerase Iota with Template U/T (pdb code 3h40). This binding sites where shown within 5.0 Angstroms radius around Bromine atom.
In total 2 binding sites of Bromine where determined in the Binary Complex of Human Dna Polymerase Iota with Template U/T, PDB code: 3h40:
Jump to Bromine binding site number: 1; 2;

Bromine binding site 1 out of 2 in the Binary Complex of Human Dna Polymerase Iota with Template U/T


Mono view


Stereo pair view

A full contact list of Bromine with other atoms in the Br binding site number 1 of Binary Complex of Human Dna Polymerase Iota with Template U/T within 5.0Å range: probe atom residue distance (Å) B Occ T:Br840 b:80.3 occ:0.50 BR T:BRU840 0.0 80.3 0.5 C5 T:BRU840 1.9 76.4 0.5 C6 T:BRU840 2.8 75.3 0.5 C4 T:BRU840 2.9 76.1 0.5 O2 T:BRU840 3.0 62.3 0.5 O4 T:BRU840 3.2 75.6 0.5 O5' T:DT839 3.3 87.8 1.0 C2' T:DT839 3.4 85.8 1.0 C3' T:DT839 3.5 84.0 1.0 N3 T:BRU840 3.6 63.5 0.5 C2 T:BRU840 3.7 64.0 0.5 C5' T:DT839 3.7 87.2 1.0 C6 T:DT839 3.9 89.7 1.0 C4' T:DT839 4.1 85.9 1.0 N1 T:BRU840 4.1 74.2 0.5 N3 T:BRU840 4.1 75.4 0.5 C1' T:DT839 4.5 87.4 1.0 C7 T:DT839 4.5 90.3 1.0 O4' T:DT839 4.6 86.9 1.0 C5 T:DT839 4.6 90.2 1.0 N1 T:DT839 4.6 88.9 1.0 C2 T:BRU840 4.7 75.6 0.5 N7 T:DG841 4.7 33.4 1.0 O3' T:DT839 4.7 81.2 1.0 C4 T:BRU840 4.8 64.1 0.5 O6 T:DG841 4.8 37.4 1.0

Bromine binding site 2 out of 2 in the Binary Complex of Human Dna Polymerase Iota with Template U/T


Mono view


Stereo pair view

A full contact list of Bromine with other atoms in the Br binding site number 2 of Binary Complex of Human Dna Polymerase Iota with Template U/T within 5.0Å range: probe atom residue distance (Å) B Occ T:Br840 b:67.3 occ:0.50 BR T:BRU840 0.0 67.3 0.5 C5 T:BRU840 1.9 64.5 0.5 O2 T:BRU840 2.7 75.2 0.5 O T:HOH227 2.8 27.8 0.5 C6 T:BRU840 2.8 64.6 0.5 C4 T:BRU840 2.9 64.1 0.5 O4 T:BRU840 3.1 63.7 0.5 O T:HOH227 3.4 54.5 0.5 C2 T:BRU840 3.5 75.6 0.5 N3 T:BRU840 3.5 75.4 0.5 O A:HOH572 3.9 45.4 1.0 O T:HOH19 4.0 32.9 1.0 N1 T:DG841 4.0 29.1 1.0 CD2 A:LEU78 4.1 80.7 1.0 N3 T:BRU840 4.1 63.5 0.5 N1 T:BRU840 4.1 63.9 0.5 CG1 A:VAL64 4.1 42.2 1.0 C2 T:DG841 4.1 29.9 1.0 CB A:VAL64 4.3 42.9 1.0 N2 T:DG841 4.3 25.8 1.0 C6 T:DG841 4.5 34.9 1.0 N3 T:DG841 4.6 30.3 1.0 OE1 A:GLN59 4.6 40.8 1.0 C2 T:BRU840 4.7 64.0 0.5 C4 T:BRU840 4.8 76.1 0.5 N1 T:BRU840 4.8 74.2 0.5 C5 T:DG841 4.9 32.4 1.0 O6 T:DG841 4.9 37.4 1.0 C4 T:DG841 4.9 33.3 1.0

R.Jain, D.T.Nair, R.E.Johnson, L.Prakash, S.Prakash, A.K.Aggarwal. Replication Across Template T/U By Human Dna Polymerase-Iota. Structure V. 17 974 2009.
ISSN: ISSN 0969-2126
PubMed: 19604477
DOI: 10.1016/J.STR.2009.04.011