Bromine in PDB 3hou: Complete Rna Polymerase II Elongation Complex I with A T-U Mismatch

All present enzymatic activity of Complete Rna Polymerase II Elongation Complex I with A T-U Mismatch:
2.7.7.6;

The structure of Complete Rna Polymerase II Elongation Complex I with A T-U Mismatch, PDB code: 3hou was solved by J.F.Sydow, F.Brueckner, A.C.M.Cheung, G.E.Damsma, S.Dengl, E.Lehmann, D.Vassylyev, P.Cramer, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	40.00 / 3.20
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	394.264, 221.614, 283.446, 90.00, 90.90, 90.00
R / Rfree (%)	23.3 / 25.2

The structure of Complete Rna Polymerase II Elongation Complex I with A T-U Mismatch also contains other interesting chemical elements:

Zinc

(Zn)

16 atoms

The binding sites of Bromine atom in the Complete Rna Polymerase II Elongation Complex I with A T-U Mismatch (pdb code 3hou). This binding sites where shown within 5.0 Angstroms radius around Bromine atom.
In total 2 binding sites of Bromine where determined in the Complete Rna Polymerase II Elongation Complex I with A T-U Mismatch, PDB code: 3hou:
Jump to Bromine binding site number: 1; 2;

Bromine binding site 1 out of 2 in the Complete Rna Polymerase II Elongation Complex I with A T-U Mismatch


Mono view


Stereo pair view

A full contact list of Bromine with other atoms in the Br binding site number 1 of Complete Rna Polymerase II Elongation Complex I with A T-U Mismatch within 5.0Å range: probe atom residue distance (Å) B Occ 1:Br23 b:85.5 occ:1.00 BR 1:BRU23 0.0 85.5 1.0 C5 1:BRU23 1.5 88.7 1.0 C6 1:BRU23 2.5 90.2 1.0 C4 1:BRU23 2.5 88.8 1.0 O4 1:BRU23 2.9 89.8 1.0 C2' 1:DC22 3.7 77.9 1.0 C6 1:DC22 3.8 76.8 1.0 N1 1:DC22 3.8 76.8 1.0 C5 1:DC22 3.8 75.5 1.0 N3 1:BRU23 3.8 89.0 1.0 N1 1:BRU23 3.8 91.2 1.0 C4 1:DC22 3.8 76.2 1.0 C2 1:DC22 3.9 76.6 1.0 N3 1:DC22 3.9 76.7 1.0 C2 1:BRU23 4.4 89.6 1.0 C1' 1:DC22 4.4 76.0 1.0 N4 1:DC22 4.5 76.6 1.0 O2 1:DC22 4.5 76.5 1.0 C5' 1:BRU23 4.7 90.4 1.0 OP2 1:BRU23 4.8 89.8 1.0 C1' 1:BRU23 5.0 92.3 1.0

Bromine binding site 2 out of 2 in the Complete Rna Polymerase II Elongation Complex I with A T-U Mismatch


Mono view


Stereo pair view

A full contact list of Bromine with other atoms in the Br binding site number 2 of Complete Rna Polymerase II Elongation Complex I with A T-U Mismatch within 5.0Å range: probe atom residue distance (Å) B Occ 4:Br23 b:83.5 occ:1.00 BR 4:BRU23 0.0 83.5 1.0 C5 4:BRU23 1.5 86.6 1.0 C6 4:BRU23 2.5 88.6 1.0 C4 4:BRU23 2.5 86.9 1.0 O4 4:BRU23 2.9 87.9 1.0 C2' 4:DC22 3.7 80.9 1.0 N1 4:DC22 3.8 79.8 1.0 C6 4:DC22 3.8 79.9 1.0 N3 4:BRU23 3.8 87.3 1.0 C5 4:DC22 3.8 78.7 1.0 N1 4:BRU23 3.8 90.1 1.0 C4 4:DC22 3.8 78.7 1.0 C2 4:DC22 3.8 79.8 1.0 N3 4:DC22 3.9 79.5 1.0 C1' 4:DC22 4.3 79.2 1.0 C2 4:BRU23 4.3 88.3 1.0 O2 4:DC22 4.5 80.3 1.0 N4 4:DC22 4.5 78.5 1.0 C5' 4:BRU23 4.7 90.9 1.0 OP2 4:BRU23 4.8 90.2 1.0 C1' 4:BRU23 5.0 91.6 1.0

J.F.Sydow, F.Brueckner, A.C.M.Cheung, G.E.Damsma, S.Dengl, E.Lehmann, D.Vassylyev, P.Cramer. Structural Basis of Transcription: Mismatch-Specific Fidelity Mechanisms and Paused Rna Polymerase II with Frayed Rna. Mol.Cell V. 34 710 2009.
ISSN: ISSN 1097-2765
PubMed: 19560423
DOI: 10.1016/J.MOLCEL.2009.06.002