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Bromine in PDB 3i4m: 8-Oxoguanine Containing Rna Polymerase II Elongation Complex D

Enzymatic activity of 8-Oxoguanine Containing Rna Polymerase II Elongation Complex D

All present enzymatic activity of 8-Oxoguanine Containing Rna Polymerase II Elongation Complex D:
2.7.7.6;

Protein crystallography data

The structure of 8-Oxoguanine Containing Rna Polymerase II Elongation Complex D, PDB code: 3i4m was solved by G.E.Damsma, P.Cramer, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 50.00 / 3.70
Space group C 2 2 21
Cell size a, b, c (Å), α, β, γ (°) 220.646, 392.003, 281.455, 90.00, 90.00, 90.00
R / Rfree (%) 22.5 / 25.8

Other elements in 3i4m:

The structure of 8-Oxoguanine Containing Rna Polymerase II Elongation Complex D also contains other interesting chemical elements:

Magnesium (Mg) 1 atom
Zinc (Zn) 8 atoms

Bromine Binding Sites:

The binding sites of Bromine atom in the 8-Oxoguanine Containing Rna Polymerase II Elongation Complex D (pdb code 3i4m). This binding sites where shown within 5.0 Angstroms radius around Bromine atom.
In total only one binding site of Bromine was determined in the 8-Oxoguanine Containing Rna Polymerase II Elongation Complex D, PDB code: 3i4m:

Bromine binding site 1 out of 1 in 3i4m

Go back to Bromine Binding Sites List in 3i4m
Bromine binding site 1 out of 1 in the 8-Oxoguanine Containing Rna Polymerase II Elongation Complex D


Mono view


Stereo pair view

A full contact list of Bromine with other atoms in the Br binding site number 1 of 8-Oxoguanine Containing Rna Polymerase II Elongation Complex D within 5.0Å range:
probe atom residue distance (Å) B Occ
T:Br23

b:0.6
occ:1.00
BR T:BRU23 0.0 0.6 1.0
C5 T:BRU23 1.5 0.7 1.0
C6 T:BRU23 2.5 0.7 1.0
C4 T:BRU23 2.5 0.6 1.0
O4 T:BRU23 2.9 0.8 1.0
C5 T:DC22 3.5 1.0 1.0
C6 T:DC22 3.7 0.6 1.0
C4 T:DC22 3.7 0.8 1.0
N3 T:BRU23 3.8 0.4 1.0
N1 T:BRU23 3.8 0.1 1.0
N1 T:DC22 4.1 0.7 1.0
N3 T:DC22 4.1 0.0 1.0
C2 T:DC22 4.3 0.2 1.0
N4 T:DC22 4.3 0.4 1.0
C2 T:BRU23 4.3 0.0 1.0
OP2 T:BRU23 4.5 0.6 1.0
C2' T:DC22 4.5 0.2 1.0
C1' T:DC22 4.9 0.6 1.0
O4' T:BRU23 4.9 0.7 1.0
C3' T:DC22 4.9 0.2 1.0
C1' T:BRU23 5.0 0.2 1.0

Reference:

G.E.Damsma, P.Cramer. Molecular Basis of Transcriptional Mutagenesis at 8-Oxoguanine J.Biol.Chem. V. 284 31658 2009.
ISSN: ISSN 0021-9258
PubMed: 19758983
DOI: 10.1074/JBC.M109.022764
Page generated: Sat Dec 12 02:12:41 2020

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