Bromine in PDB 3i4m: 8-Oxoguanine Containing Rna Polymerase II Elongation Complex D

All present enzymatic activity of 8-Oxoguanine Containing Rna Polymerase II Elongation Complex D:
2.7.7.6;

The structure of 8-Oxoguanine Containing Rna Polymerase II Elongation Complex D, PDB code: 3i4m was solved by G.E.Damsma, P.Cramer, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	50.00 / 3.70
Space group	C 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	220.646, 392.003, 281.455, 90.00, 90.00, 90.00
R / Rfree (%)	22.5 / 25.8

The structure of 8-Oxoguanine Containing Rna Polymerase II Elongation Complex D also contains other interesting chemical elements:

Magnesium	(Mg)	1 atom
Zinc	(Zn)	8 atoms

The binding sites of Bromine atom in the 8-Oxoguanine Containing Rna Polymerase II Elongation Complex D (pdb code 3i4m). This binding sites where shown within 5.0 Angstroms radius around Bromine atom.
In total only one binding site of Bromine was determined in the 8-Oxoguanine Containing Rna Polymerase II Elongation Complex D, PDB code: 3i4m:

Bromine binding site 1 out of 1 in the 8-Oxoguanine Containing Rna Polymerase II Elongation Complex D


Mono view


Stereo pair view

A full contact list of Bromine with other atoms in the Br binding site number 1 of 8-Oxoguanine Containing Rna Polymerase II Elongation Complex D within 5.0Å range: probe atom residue distance (Å) B Occ T:Br23 b:0.6 occ:1.00 BR T:BRU23 0.0 0.6 1.0 C5 T:BRU23 1.5 0.7 1.0 C6 T:BRU23 2.5 0.7 1.0 C4 T:BRU23 2.5 0.6 1.0 O4 T:BRU23 2.9 0.8 1.0 C5 T:DC22 3.5 1.0 1.0 C6 T:DC22 3.7 0.6 1.0 C4 T:DC22 3.7 0.8 1.0 N3 T:BRU23 3.8 0.4 1.0 N1 T:BRU23 3.8 0.1 1.0 N1 T:DC22 4.1 0.7 1.0 N3 T:DC22 4.1 0.0 1.0 C2 T:DC22 4.3 0.2 1.0 N4 T:DC22 4.3 0.4 1.0 C2 T:BRU23 4.3 0.0 1.0 OP2 T:BRU23 4.5 0.6 1.0 C2' T:DC22 4.5 0.2 1.0 C1' T:DC22 4.9 0.6 1.0 O4' T:BRU23 4.9 0.7 1.0 C3' T:DC22 4.9 0.2 1.0 C1' T:BRU23 5.0 0.2 1.0

G.E.Damsma, P.Cramer. Molecular Basis of Transcriptional Mutagenesis at 8-Oxoguanine J.Biol.Chem. V. 284 31658 2009.
ISSN: ISSN 0021-9258
PubMed: 19758983
DOI: 10.1074/JBC.M109.022764