Bromine in PDB 4fbm: Lips and Lipt, Two Metagenome-Derived Lipolytic Enzymes Increase the Diversity of Known Lipase and Esterase Families

The structure of Lips and Lipt, Two Metagenome-Derived Lipolytic Enzymes Increase the Diversity of Known Lipase and Esterase Families, PDB code: 4fbm was solved by J.Chow, U.Krauss, Y.Dall Antonia, F.Fersini, C.Schmeisser, M.Schmidt, I.Menyes, U.Bornscheuer, B.Lauinger, P.Bongen, J.Pietruszka, M.Eckstein, O.Thum, A.Liese, J.Mueller-Dieckmann, K.-E.Jaeger, F.Kovavic, W.R.Streit, Structural Proteomics In Europe (Spine), with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	46.30 / 2.80
Space group	P 42 21 2
Cell size a, b, c (Å), α, β, γ (°)	170.650, 170.650, 46.830, 90.00, 90.00, 90.00
R / Rfree (%)	22.3 / 27

The binding sites of Bromine atom in the Lips and Lipt, Two Metagenome-Derived Lipolytic Enzymes Increase the Diversity of Known Lipase and Esterase Families (pdb code 4fbm). This binding sites where shown within 5.0 Angstroms radius around Bromine atom.
In total 2 binding sites of Bromine where determined in the Lips and Lipt, Two Metagenome-Derived Lipolytic Enzymes Increase the Diversity of Known Lipase and Esterase Families, PDB code: 4fbm:
Jump to Bromine binding site number: 1; 2;

Bromine binding site 1 out of 2 in the Lips and Lipt, Two Metagenome-Derived Lipolytic Enzymes Increase the Diversity of Known Lipase and Esterase Families


Mono view


Stereo pair view

A full contact list of Bromine with other atoms in the Br binding site number 1 of Lips and Lipt, Two Metagenome-Derived Lipolytic Enzymes Increase the Diversity of Known Lipase and Esterase Families within 5.0Å range: probe atom residue distance (Å) B Occ A:Br301 b:40.3 occ:1.00 N A:GLU141 3.4 19.6 1.0 C A:PHE139 3.5 16.4 1.0 CA A:PHE139 3.6 15.6 1.0 CB A:GLU141 3.7 21.1 1.0 N A:PRO140 3.7 17.1 1.0 O A:PHE139 3.8 16.5 1.0 CD A:PRO140 3.8 17.3 1.0 N A:ARG142 3.9 18.6 1.0 CB A:PHE139 3.9 14.9 1.0 CA A:GLU141 4.0 20.2 1.0 CB A:ARG142 4.2 17.6 1.0 C A:GLU141 4.3 19.3 1.0 C A:PRO140 4.4 19.1 1.0 CG A:GLU141 4.4 25.0 1.0 CA A:ARG142 4.6 17.4 1.0 CA A:PRO140 4.6 17.9 1.0 CD2 A:PHE139 4.7 14.2 1.0 CG A:PHE139 4.8 14.6 1.0 CB A:PRO140 4.9 18.1 1.0 CG A:PRO140 4.9 17.6 1.0 N A:PHE139 4.9 15.3 1.0

Bromine binding site 2 out of 2 in the Lips and Lipt, Two Metagenome-Derived Lipolytic Enzymes Increase the Diversity of Known Lipase and Esterase Families


Mono view


Stereo pair view

A full contact list of Bromine with other atoms in the Br binding site number 2 of Lips and Lipt, Two Metagenome-Derived Lipolytic Enzymes Increase the Diversity of Known Lipase and Esterase Families within 5.0Å range: probe atom residue distance (Å) B Occ B:Br301 b:37.7 occ:1.00 N B:GLU141 3.5 23.6 1.0 C B:PHE139 3.6 18.2 1.0 CA B:PHE139 3.7 16.7 1.0 CD B:PRO140 3.7 19.6 1.0 N B:PRO140 3.8 19.6 1.0 CB B:GLU141 3.8 25.4 1.0 N B:ARG142 3.9 23.8 1.0 CB B:PHE139 3.9 15.2 1.0 CG B:GLU141 4.0 30.1 1.0 CA B:GLU141 4.0 24.7 1.0 O B:PHE139 4.0 17.7 1.0 C B:GLU141 4.2 24.1 1.0 C B:PRO140 4.5 22.2 1.0 NE B:ARG142 4.5 37.2 1.0 CB B:ARG142 4.6 24.0 1.0 CA B:PRO140 4.6 21.0 1.0 CA B:ARG142 4.6 23.4 1.0 CD1 B:PHE139 4.7 10.2 1.0 CG B:PRO140 4.8 20.6 1.0 CG B:PHE139 4.8 12.0 1.0 CB B:PRO140 4.9 20.8 1.0

J.Chow, F.Kovacic, Y.Dall Antonia, U.Krauss, F.Fersini, C.Schmeisser, B.Lauinger, P.Bongen, J.Pietruszka, M.Schmidt, I.Menyes, U.T.Bornscheuer, M.Eckstein, O.Thum, A.Liese, J.Mueller-Dieckmann, K.E.Jaeger, W.R.Streit. The Metagenome-Derived Enzymes Lips and Lipt Increase the Diversity of Known Lipases. Plos One V. 7 47665 2012.
ISSN: ESSN 1932-6203
PubMed: 23112831
DOI: 10.1371/JOURNAL.PONE.0047665