Bromine in PDB 6syc: Crystal Structure of the Lysozyme in Presence of Bromophenol Blue at pH 6.5

All present enzymatic activity of Crystal Structure of the Lysozyme in Presence of Bromophenol Blue at pH 6.5:
3.2.1.17;

The structure of Crystal Structure of the Lysozyme in Presence of Bromophenol Blue at pH 6.5, PDB code: 6syc was solved by A.Camara-Artigas, M.Plaza-Garrido, M.C.Salinas-Garcia, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	19.39 / 1.38
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	37.719, 76.559, 84.076, 90.00, 90.00, 90.00
R / Rfree (%)	19.1 / 22.6

The structure of Crystal Structure of the Lysozyme in Presence of Bromophenol Blue at pH 6.5 also contains other interesting chemical elements:

Chlorine	(Cl)	1 atom
Sodium	(Na)	2 atoms

Pages:

>>> Page 1 <<< Page 2, Binding sites: 11 - 20; Page 3, Binding sites: 21 - 30; Page 4, Binding sites: 31 - 32;

Binding sites:

The binding sites of Bromine atom in the Crystal Structure of the Lysozyme in Presence of Bromophenol Blue at pH 6.5 (pdb code 6syc). This binding sites where shown within 5.0 Angstroms radius around Bromine atom.
In total 32 binding sites of Bromine where determined in the Crystal Structure of the Lysozyme in Presence of Bromophenol Blue at pH 6.5, PDB code: 6syc:
Jump to Bromine binding site number: 1; 2; 3; 4; 5; 6; 7; 8; 9; 10;

Bromine binding site 1 out of 32 in the Crystal Structure of the Lysozyme in Presence of Bromophenol Blue at pH 6.5


Mono view


Stereo pair view

A full contact list of Bromine with other atoms in the Br binding site number 1 of Crystal Structure of the Lysozyme in Presence of Bromophenol Blue at pH 6.5 within 5.0Å range: probe atom residue distance (Å) B Occ A:Br202 b:20.6 occ:1.00 BR3 A:LYE202 0.0 20.6 1.0 C13 A:LYE202 1.9 16.8 1.0 C11 A:LYE202 2.8 17.9 1.0 C14 A:LYE202 2.9 16.0 1.0 HB3 B:SER100 2.9 31.4 1.0 H141 A:LYE202 3.0 19.2 1.0 O12 A:LYE202 3.0 18.5 1.0 HA B:LYS97 3.1 11.7 1.0 OH B:TYR20 3.3 18.4 1.0 HH B:TYR20 3.4 22.0 1.0 HG2 B:LYS97 3.4 14.9 1.0 HG B:SER100 3.5 35.0 1.0 BR4 B:LYE203 3.5 20.2 1.0 CB B:SER100 3.6 26.2 1.0 HB3 B:LYS96 3.6 15.6 1.0 HB2 B:SER100 3.6 31.4 1.0 O B:LYS96 3.7 11.1 1.0 H221 B:LYE203 3.8 15.2 1.0 HG3 B:LYS97 3.9 14.9 1.0 OG B:SER100 4.0 29.1 1.0 BR2 B:LYE203 4.0 15.2 1.0 CA B:LYS97 4.0 9.7 1.0 C B:LYS96 4.0 9.8 1.0 HG21 A:THR47 4.0 22.1 1.0 H091 B:LYE203 4.0 17.6 1.0 CG B:LYS97 4.0 12.4 1.0 N B:LYS97 4.1 9.9 1.0 C10 A:LYE202 4.1 18.2 1.0 C08 A:LYE202 4.2 15.3 1.0 C10 B:LYE203 4.2 16.7 1.0 HG22 A:THR47 4.3 22.1 1.0 C09 B:LYE203 4.4 14.6 1.0 HG23 A:THR47 4.4 22.1 1.0 HB2 B:LYS96 4.4 15.6 1.0 CB B:LYS96 4.4 13.0 1.0 CG2 A:THR47 4.5 18.4 1.0 CZ B:TYR20 4.6 14.8 1.0 C22 B:LYE203 4.6 12.7 1.0 H B:LYS97 4.6 11.9 1.0 CB B:LYS97 4.6 11.8 1.0 C09 A:LYE202 4.7 16.1 1.0 H221 A:LYE202 4.7 18.1 1.0 H B:SER100 4.7 23.4 1.0 C21 B:LYE203 4.8 13.9 1.0 HE1 B:TYR20 4.8 17.2 1.0 HE2 B:LYS97 4.8 20.1 1.0 CA B:LYS96 4.9 10.9 1.0 CA B:SER100 5.0 23.4 1.0 HB3 B:LYS97 5.0 14.2 1.0

Bromine binding site 2 out of 32 in the Crystal Structure of the Lysozyme in Presence of Bromophenol Blue at pH 6.5


Mono view


Stereo pair view

A full contact list of Bromine with other atoms in the Br binding site number 2 of Crystal Structure of the Lysozyme in Presence of Bromophenol Blue at pH 6.5 within 5.0Å range: probe atom residue distance (Å) B Occ A:Br202 b:22.7 occ:1.00 BR4 A:LYE202 0.0 22.7 1.0 C10 A:LYE202 1.9 18.2 1.0 C11 A:LYE202 2.8 17.9 1.0 C09 A:LYE202 2.9 16.1 1.0 H091 A:LYE202 3.0 19.3 1.0 H181 B:LYE205 3.0 23.1 1.0 O12 A:LYE202 3.0 18.5 1.0 C10 B:LYE205 3.5 16.5 1.0 C11 B:LYE205 3.6 16.5 1.0 C09 B:LYE205 3.8 15.6 1.0 C13 B:LYE205 3.9 17.8 1.0 HE2 B:LYS97 3.9 20.1 1.0 C08 B:LYE205 4.1 17.4 1.0 C18 B:LYE205 4.1 19.3 1.0 O12 B:LYE205 4.1 16.4 1.0 HD3 B:LYS97 4.1 19.0 1.0 C13 A:LYE202 4.1 16.8 1.0 C14 B:LYE205 4.1 17.4 1.0 C08 A:LYE202 4.2 15.3 1.0 H091 B:LYE205 4.2 18.8 1.0 HG22 A:THR47 4.2 22.1 1.0 HG2 B:LYS97 4.2 14.9 1.0 BR4 B:LYE205 4.3 19.1 1.0 HZ2 B:LYS97 4.4 23.5 1.0 HG21 A:THR47 4.6 22.1 1.0 BR1 B:LYE205 4.6 21.8 1.0 HB A:THR47 4.7 21.7 1.0 C14 A:LYE202 4.7 16.0 1.0 CE B:LYS97 4.7 16.7 1.0 CD B:LYS97 4.7 15.9 1.0 CG2 A:THR47 4.8 18.4 1.0 H141 B:LYE205 4.8 20.9 1.0 C07 B:LYE205 4.9 18.3 1.0 C19 B:LYE205 4.9 20.2 1.0 O27 A:LYE202 4.9 22.9 1.0 C17 B:LYE205 5.0 18.2 1.0 CG B:LYS97 5.0 12.4 1.0 BR3 B:LYE205 5.0 21.9 1.0

Bromine binding site 3 out of 32 in the Crystal Structure of the Lysozyme in Presence of Bromophenol Blue at pH 6.5


Mono view


Stereo pair view

A full contact list of Bromine with other atoms in the Br binding site number 3 of Crystal Structure of the Lysozyme in Presence of Bromophenol Blue at pH 6.5 within 5.0Å range: probe atom residue distance (Å) B Occ A:Br202 b:25.9 occ:1.00 BR2 A:LYE202 0.0 25.9 1.0 C21 A:LYE202 1.9 19.1 1.0 HH11 A:ARG45 2.7 26.6 1.0 C20 A:LYE202 2.8 19.1 1.0 C22 A:LYE202 2.9 15.1 1.0 O24 A:LYE202 3.0 19.9 1.0 H221 A:LYE202 3.0 18.1 1.0 HB3 A:ARG45 3.0 16.2 1.0 O A:ARG45 3.1 15.4 1.0 HG2 A:ARG45 3.2 20.4 1.0 NH1 A:ARG45 3.4 22.1 1.0 C A:ARG45 3.5 14.4 1.0 HD3 A:ARG45 3.5 24.6 1.0 H091 B:LYE203 3.5 17.6 1.0 C10 B:LYE203 3.6 16.7 1.0 HH12 A:ARG45 3.6 26.6 1.0 C09 B:LYE203 3.6 14.6 1.0 BR4 B:LYE203 3.7 20.2 1.0 H061 B:LYE203 3.7 15.7 1.0 CB A:ARG45 3.7 13.5 1.0 CG A:ARG45 3.8 17.0 1.0 HA A:ASN46 3.9 17.7 1.0 N A:ASN46 4.0 13.9 1.0 C19 A:LYE202 4.1 18.9 1.0 CD A:ARG45 4.1 20.5 1.0 C17 A:LYE202 4.2 15.5 1.0 C A:ASN46 4.2 15.7 1.0 CA A:ARG45 4.2 12.5 1.0 O A:ASN46 4.2 16.5 1.0 CA A:ASN46 4.3 14.8 1.0 C11 B:LYE203 4.3 15.9 1.0 C08 B:LYE203 4.4 13.4 1.0 CZ A:ARG45 4.5 24.1 1.0 H A:ARG45 4.5 14.4 1.0 HG23 A:THR47 4.5 22.1 1.0 HB2 A:ARG45 4.6 16.2 1.0 H011 A:LYE203 4.6 31.4 1.0 HA A:THR47 4.6 22.1 1.0 H A:ASN46 4.6 16.6 1.0 C18 A:LYE202 4.6 16.8 1.0 N A:THR47 4.7 16.6 1.0 HG3 A:ARG45 4.7 20.4 1.0 N A:ARG45 4.7 12.0 1.0 H141 A:LYE202 4.8 19.2 1.0 NE A:ARG45 4.8 24.1 1.0 C06 B:LYE203 4.8 13.1 1.0 O12 B:LYE203 4.9 17.5 1.0 HD2 A:ARG45 4.9 24.6 1.0 O28 A:LYE202 4.9 21.5 1.0 H A:THR47 4.9 19.9 1.0 C13 B:LYE203 5.0 14.5 1.0 C14 B:LYE203 5.0 14.1 1.0

Bromine binding site 4 out of 32 in the Crystal Structure of the Lysozyme in Presence of Bromophenol Blue at pH 6.5


Mono view


Stereo pair view

A full contact list of Bromine with other atoms in the Br binding site number 4 of Crystal Structure of the Lysozyme in Presence of Bromophenol Blue at pH 6.5 within 5.0Å range: probe atom residue distance (Å) B Occ A:Br202 b:19.0 occ:1.00 BR1 A:LYE202 0.0 19.0 1.0 C19 A:LYE202 1.9 18.9 1.0 C20 A:LYE202 2.8 19.1 1.0 C18 A:LYE202 2.9 16.8 1.0 H221 A:LYE203 2.9 20.4 1.0 O24 A:LYE202 3.0 19.9 1.0 H181 A:LYE202 3.0 20.2 1.0 H061 A:LYE203 3.2 28.1 1.0 H181 B:LYE203 3.2 16.6 1.0 C22 A:LYE203 3.5 17.0 1.0 C06 A:LYE203 3.6 23.5 1.0 H061 B:LYE203 3.7 15.7 1.0 C18 B:LYE203 3.8 13.8 1.0 C06 B:LYE203 3.9 13.1 1.0 H021 B:LYE208 3.9 16.9 1.0 C21 A:LYE203 4.0 18.4 1.0 BR2 A:LYE203 4.0 21.4 1.0 C21 A:LYE202 4.1 19.1 1.0 C01 B:LYE203 4.1 14.0 1.0 C01 A:LYE203 4.1 26.2 1.0 H011 B:LYE203 4.1 16.8 1.0 C17 A:LYE202 4.2 15.5 1.0 H011 A:LYE203 4.2 31.4 1.0 C05 A:LYE203 4.2 21.8 1.0 C17 A:LYE203 4.2 17.6 1.0 BR1 B:LYE203 4.2 19.0 1.0 C19 B:LYE203 4.3 13.2 1.0 C07 A:LYE203 4.4 18.0 1.0 C05 B:LYE203 4.5 12.7 1.0 C17 B:LYE203 4.6 13.1 1.0 C22 A:LYE202 4.6 15.1 1.0 C02 B:LYE208 4.7 14.1 1.0 C07 B:LYE203 4.9 12.5 1.0 C02 B:LYE203 5.0 14.9 1.0 O28 A:LYE202 5.0 21.5 1.0

Bromine binding site 5 out of 32 in the Crystal Structure of the Lysozyme in Presence of Bromophenol Blue at pH 6.5


Mono view


Stereo pair view

A full contact list of Bromine with other atoms in the Br binding site number 5 of Crystal Structure of the Lysozyme in Presence of Bromophenol Blue at pH 6.5 within 5.0Å range: probe atom residue distance (Å) B Occ A:Br203 b:16.2 occ:1.00 BR3 A:LYE203 0.0 16.2 1.0 C13 A:LYE203 1.9 15.7 1.0 C14 A:LYE203 2.8 14.9 1.0 C11 A:LYE203 2.8 14.5 1.0 H141 A:LYE203 2.9 17.9 1.0 O12 A:LYE203 3.0 13.4 1.0 H031 A:LYE202 3.4 20.8 1.0 BR4 B:LYE206 3.6 17.9 1.0 C10 A:LYE203 4.1 16.1 1.0 C08 A:LYE203 4.1 16.0 1.0 O29 A:LYE202 4.2 24.5 1.0 C03 A:LYE202 4.4 17.4 1.0 H021 A:LYE202 4.7 19.8 1.0 C09 A:LYE203 4.7 16.3 1.0

Bromine binding site 6 out of 32 in the Crystal Structure of the Lysozyme in Presence of Bromophenol Blue at pH 6.5


Mono view


Stereo pair view

A full contact list of Bromine with other atoms in the Br binding site number 6 of Crystal Structure of the Lysozyme in Presence of Bromophenol Blue at pH 6.5 within 5.0Å range: probe atom residue distance (Å) B Occ A:Br203 b:16.6 occ:1.00 BR4 A:LYE203 0.0 16.6 1.0 C10 A:LYE203 1.9 16.1 1.0 C11 A:LYE203 2.8 14.5 1.0 C09 A:LYE203 2.9 16.3 1.0 H091 A:LYE203 3.0 19.5 1.0 H011 B:LYE208 3.0 16.6 1.0 O12 A:LYE203 3.0 13.4 1.0 H021 A:LYE202 3.2 19.8 1.0 H141 B:LYE204 3.2 34.2 1.0 O A:HOH308 3.3 25.3 1.0 C02 B:LYE204 3.7 16.2 1.0 C03 B:LYE204 3.7 17.5 1.0 C02 A:LYE202 3.9 16.5 1.0 C01 B:LYE208 4.0 13.8 1.0 C01 B:LYE204 4.0 16.1 1.0 C04 B:LYE204 4.0 17.9 1.0 H021 B:LYE204 4.0 19.5 1.0 H031 B:LYE204 4.0 21.0 1.0 H011 A:LYE202 4.1 19.8 1.0 C13 A:LYE203 4.2 15.7 1.0 C08 A:LYE203 4.2 16.0 1.0 C14 B:LYE204 4.2 28.5 1.0 C06 B:LYE204 4.2 15.5 1.0 C05 B:LYE204 4.3 17.3 1.0 H061 B:LYE208 4.3 15.2 1.0 C01 A:LYE202 4.3 16.5 1.0 H181 A:LYE203 4.4 21.2 1.0 H011 B:LYE204 4.4 19.4 1.0 BR3 B:LYE204 4.5 32.1 1.0 C06 B:LYE208 4.6 12.7 1.0 C14 A:LYE203 4.7 14.9 1.0 O28 B:LYE204 4.8 21.8 1.0 O27 B:LYE204 4.8 22.1 1.0 C13 B:LYE204 4.8 30.9 1.0 C03 A:LYE202 4.9 17.4 1.0 S26 B:LYE204 4.9 21.4 1.0 H061 B:LYE204 4.9 18.6 1.0 C02 B:LYE208 4.9 14.1 1.0 H021 B:LYE208 4.9 16.9 1.0

Bromine binding site 7 out of 32 in the Crystal Structure of the Lysozyme in Presence of Bromophenol Blue at pH 6.5


Mono view


Stereo pair view

A full contact list of Bromine with other atoms in the Br binding site number 7 of Crystal Structure of the Lysozyme in Presence of Bromophenol Blue at pH 6.5 within 5.0Å range: probe atom residue distance (Å) B Occ A:Br203 b:21.4 occ:1.00 BR2 A:LYE203 0.0 21.4 1.0 C21 A:LYE203 1.9 18.4 1.0 C22 A:LYE203 2.8 17.0 1.0 C20 A:LYE203 2.9 16.5 1.0 H221 A:LYE203 2.9 20.4 1.0 O24 A:LYE203 3.1 17.5 1.0 BR1 A:LYE202 4.0 19.0 1.0 H031 B:LYE208 4.0 18.1 1.0 C17 A:LYE203 4.2 17.6 1.0 C19 A:LYE203 4.2 18.9 1.0 H021 B:LYE208 4.5 16.9 1.0 O28 A:LYE203 4.6 40.0 1.0 H011 B:LYE203 4.7 16.8 1.0 C18 A:LYE203 4.7 17.6 1.0 H021 B:LYE203 5.0 17.9 1.0 C03 B:LYE208 5.0 15.1 1.0

Bromine binding site 8 out of 32 in the Crystal Structure of the Lysozyme in Presence of Bromophenol Blue at pH 6.5


Mono view


Stereo pair view

A full contact list of Bromine with other atoms in the Br binding site number 8 of Crystal Structure of the Lysozyme in Presence of Bromophenol Blue at pH 6.5 within 5.0Å range: probe atom residue distance (Å) B Occ A:Br203 b:22.8 occ:1.00 BR1 A:LYE203 0.0 22.8 1.0 C19 A:LYE203 1.9 18.9 1.0 C18 A:LYE203 2.8 17.6 1.0 C20 A:LYE203 2.9 16.5 1.0 H181 A:LYE203 2.9 21.2 1.0 O24 A:LYE203 3.1 17.5 1.0 H011 B:LYE204 3.9 19.4 1.0 C02 B:LYE208 4.0 14.1 1.0 H021 B:LYE208 4.0 16.9 1.0 C03 B:LYE208 4.1 15.1 1.0 C17 A:LYE203 4.1 17.6 1.0 H031 B:LYE208 4.1 18.1 1.0 C21 A:LYE203 4.2 18.4 1.0 H221 B:LYE208 4.2 15.9 1.0 H091 A:LYE203 4.4 19.5 1.0 C01 B:LYE208 4.6 13.8 1.0 C22 A:LYE203 4.7 17.0 1.0 C04 B:LYE208 4.7 13.3 1.0 BR2 B:LYE208 4.8 14.7 1.0 C01 B:LYE204 4.9 16.1 1.0 H011 B:LYE208 5.0 16.6 1.0

Bromine binding site 9 out of 32 in the Crystal Structure of the Lysozyme in Presence of Bromophenol Blue at pH 6.5


Mono view


Stereo pair view

A full contact list of Bromine with other atoms in the Br binding site number 9 of Crystal Structure of the Lysozyme in Presence of Bromophenol Blue at pH 6.5 within 5.0Å range: probe atom residue distance (Å) B Occ B:Br203 b:17.7 occ:1.00 BR3 B:LYE203 0.0 17.7 1.0 C13 B:LYE203 1.9 14.5 1.0 C11 B:LYE203 2.8 15.9 1.0 C14 B:LYE203 2.9 14.1 1.0 HN1 A:IMD204 3.0 29.1 1.0 O12 B:LYE203 3.0 17.5 1.0 H141 B:LYE203 3.0 16.9 1.0 H5 A:IMD204 3.1 28.7 1.0 O B:HOH367 3.3 23.8 1.0 H B:ARG21 3.5 16.3 1.0 N1 A:IMD204 3.5 24.2 1.0 C5 A:IMD204 3.6 23.9 1.0 HD1 B:TYR20 3.8 17.4 1.0 HE1 B:TYR20 3.8 17.2 1.0 CD1 B:TYR20 4.0 14.5 1.0 CE1 B:TYR20 4.0 14.3 1.0 HG2 B:ARG21 4.1 28.4 1.0 OD1 A:ASN44 4.1 16.9 1.0 O B:HOH377 4.1 27.8 1.0 C10 B:LYE203 4.1 16.7 1.0 HA B:TYR20 4.1 15.6 1.0 HH21 B:ARG21 4.2 42.1 1.0 C08 B:LYE203 4.2 13.4 1.0 HB3 A:ASN44 4.2 17.0 1.0 N B:ARG21 4.2 13.6 1.0 O A:HOH339 4.3 26.3 1.0 CG A:ASN44 4.5 16.0 1.0 HA B:ARG21 4.6 17.4 1.0 C09 B:LYE203 4.7 14.6 1.0 HD3 B:ARG21 4.7 34.6 1.0 CG B:TYR20 4.8 13.2 1.0 C2 A:IMD204 4.8 24.3 1.0 CZ B:TYR20 4.9 14.8 1.0 CB A:ASN44 4.9 14.2 1.0 NH2 B:ARG21 4.9 35.1 1.0 CA B:TYR20 4.9 13.0 1.0 C4 A:IMD204 5.0 23.1 1.0 CA B:ARG21 5.0 14.5 1.0 CG B:ARG21 5.0 23.6 1.0

Bromine binding site 10 out of 32 in the Crystal Structure of the Lysozyme in Presence of Bromophenol Blue at pH 6.5


Mono view


Stereo pair view

A full contact list of Bromine with other atoms in the Br binding site number 10 of Crystal Structure of the Lysozyme in Presence of Bromophenol Blue at pH 6.5 within 5.0Å range: probe atom residue distance (Å) B Occ B:Br203 b:20.2 occ:1.00 BR4 B:LYE203 0.0 20.2 1.0 C10 B:LYE203 1.9 16.7 1.0 C11 B:LYE203 2.8 15.9 1.0 C09 B:LYE203 2.9 14.6 1.0 HG23 A:THR47 2.9 22.1 1.0 O12 B:LYE203 3.0 17.5 1.0 H091 B:LYE203 3.0 17.6 1.0 HH22 B:ARG21 3.1 42.1 1.0 H221 A:LYE202 3.4 18.1 1.0 BR3 A:LYE202 3.5 20.6 1.0 H141 A:LYE202 3.6 19.2 1.0 CG2 A:THR47 3.7 18.4 1.0 BR2 A:LYE202 3.7 25.9 1.0 HG21 A:THR47 3.8 22.1 1.0 NH2 B:ARG21 3.9 35.1 1.0 HG22 A:THR47 3.9 22.1 1.0 HA A:ASN46 3.9 17.7 1.0 C13 A:LYE202 4.0 16.8 1.0 C14 A:LYE202 4.0 16.0 1.0 C22 A:LYE202 4.1 15.1 1.0 H A:THR47 4.1 19.9 1.0 C13 B:LYE203 4.1 14.5 1.0 HH21 B:ARG21 4.1 42.1 1.0 O A:HOH359 4.2 27.5 1.0 OH B:TYR20 4.2 18.4 1.0 C08 B:LYE203 4.2 13.4 1.0 C21 A:LYE202 4.3 19.1 1.0 HB2 B:SER100 4.3 31.4 1.0 O A:ARG45 4.4 15.4 1.0 N A:THR47 4.4 16.6 1.0 HH12 B:ARG21 4.5 42.5 1.0 HN1 A:IMD204 4.5 29.1 1.0 HD22 A:ASN46 4.6 26.4 1.0 HB3 B:SER100 4.6 31.4 1.0 H221 B:LYE203 4.6 15.2 1.0 HH B:TYR20 4.6 22.0 1.0 HE1 B:TYR20 4.6 17.2 1.0 C14 B:LYE203 4.7 14.1 1.0 CA A:ASN46 4.8 14.8 1.0 C A:ASN46 4.8 15.7 1.0 CB B:SER100 4.9 26.2 1.0 CZ B:ARG21 4.9 34.2 1.0 CB A:THR47 4.9 18.1 1.0 HA A:THR47 5.0 22.1 1.0 HG1 A:THR47 5.0 23.8 1.0

M.Plaza-Garrido, M.C.Salinas-Garcia, D.Alba-Elena, J.C.Martinez, A.Camara-Artigas. Lysozyme Crystals Dyed with Bromophenol Blue: Where Has the Dye Gone? Acta Crystallogr D Struct V. 76 845 2020BIOL.
ISSN: ISSN 2059-7983
PubMed: 32876060
DOI: 10.1107/S2059798320008803