Bromine in PDB 1azf: Chicken Egg White Lysozyme Crystal Grown in Bromide Solution

All present enzymatic activity of Chicken Egg White Lysozyme Crystal Grown in Bromide Solution:
3.2.1.17;

The structure of Chicken Egg White Lysozyme Crystal Grown in Bromide Solution, PDB code: 1azf was solved by K.Lim, A.Nadarajah, E.L.Forsythe, M.L.Pusey, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	6.00 / 1.80
Space group	P 43 21 2
Cell size a, b, c (Å), α, β, γ (°)	79.140, 79.140, 38.020, 90.00, 90.00, 90.00
R / Rfree (%)	19.3 / 24

The binding sites of Bromine atom in the Chicken Egg White Lysozyme Crystal Grown in Bromide Solution (pdb code 1azf). This binding sites where shown within 5.0 Angstroms radius around Bromine atom.
In total 4 binding sites of Bromine where determined in the Chicken Egg White Lysozyme Crystal Grown in Bromide Solution, PDB code: 1azf:
Jump to Bromine binding site number: 1; 2; 3; 4;

Bromine binding site 1 out of 4 in the Chicken Egg White Lysozyme Crystal Grown in Bromide Solution


Mono view


Stereo pair view

A full contact list of Bromine with other atoms in the Br binding site number 1 of Chicken Egg White Lysozyme Crystal Grown in Bromide Solution within 5.0Å range: probe atom residue distance (Å) B Occ A:Br286 b:41.6 occ:1.00 OH A:TYR23 3.2 19.7 1.0 O A:HOH266 3.4 53.7 1.0 CE2 A:TYR23 3.7 17.0 1.0 O A:HOH209 3.7 20.5 1.0 O A:HOH265 3.8 39.2 1.0 CZ A:TYR23 3.8 19.0 1.0 CA A:GLY104 4.2 23.9 1.0 O A:ARG21 4.5 28.9 1.0 N A:GLY104 4.8 25.6 1.0 CD2 A:TYR23 4.9 15.9 1.0

Bromine binding site 2 out of 4 in the Chicken Egg White Lysozyme Crystal Grown in Bromide Solution


Mono view


Stereo pair view

A full contact list of Bromine with other atoms in the Br binding site number 2 of Chicken Egg White Lysozyme Crystal Grown in Bromide Solution within 5.0Å range: probe atom residue distance (Å) B Occ A:Br287 b:46.3 occ:1.00 OG A:SER24 3.2 23.0 1.0 N A:GLY26 3.2 19.4 1.0 CA A:GLY26 3.5 16.7 1.0 CB A:SER24 3.5 22.0 1.0 CD1 A:ILE124 3.6 34.4 1.0 CA A:GLN121 3.8 32.4 1.0 CG1 A:ILE124 4.1 30.4 1.0 N A:GLN121 4.1 31.8 1.0 CG A:GLN121 4.2 49.4 1.0 O A:VAL120 4.3 28.6 1.0 N A:LEU25 4.4 22.2 1.0 C A:VAL120 4.4 32.3 1.0 CG2 A:VAL120 4.4 21.8 1.0 CB A:GLN121 4.4 39.5 1.0 C A:LEU25 4.5 22.1 1.0 C A:SER24 4.5 22.8 1.0 CA A:SER24 4.6 21.4 1.0 C A:GLY26 4.7 21.2 1.0 CA A:LEU25 4.9 24.0 1.0 C A:GLN121 4.9 28.7 1.0 N A:ASN27 4.9 18.7 1.0

Bromine binding site 3 out of 4 in the Chicken Egg White Lysozyme Crystal Grown in Bromide Solution


Mono view


Stereo pair view

A full contact list of Bromine with other atoms in the Br binding site number 3 of Chicken Egg White Lysozyme Crystal Grown in Bromide Solution within 5.0Å range: probe atom residue distance (Å) B Occ A:Br288 b:77.8 occ:1.00 NH2 A:ARG14 3.3 49.9 1.0 N A:ILE88 3.5 27.3 1.0 NE2 A:HIS15 3.6 31.4 1.0 OD1 A:ASP87 3.7 32.6 1.0 CG1 A:ILE88 3.8 22.4 1.0 CA A:ASP87 4.0 27.4 1.0 C A:ASP87 4.2 28.8 1.0 CG A:ASP87 4.3 34.6 1.0 O A:SER86 4.4 29.0 1.0 CB A:ALA11 4.4 23.0 1.0 CD2 A:HIS15 4.4 30.2 1.0 CA A:ILE88 4.5 23.3 1.0 CB A:ILE88 4.5 22.9 1.0 CD1 A:ILE88 4.5 24.7 1.0 CE1 A:HIS15 4.6 31.8 1.0 CG2 A:ILE88 4.6 20.5 1.0 CZ A:ARG14 4.6 47.1 1.0 CB A:ASP87 4.7 29.9 1.0 CZ A:PHE3 4.8 20.8 1.0 N A:THR89 4.9 23.3 1.0 OD2 A:ASP87 5.0 38.2 1.0

Bromine binding site 4 out of 4 in the Chicken Egg White Lysozyme Crystal Grown in Bromide Solution


Mono view


Stereo pair view

A full contact list of Bromine with other atoms in the Br binding site number 4 of Chicken Egg White Lysozyme Crystal Grown in Bromide Solution within 5.0Å range: probe atom residue distance (Å) B Occ A:Br289 b:71.3 occ:1.00 O A:HOH251 3.3 38.1 1.0 CE2 A:PHE38 3.9 22.7 1.0 NZ A:LYS33 4.1 33.7 1.0 CD A:ARG5 4.3 28.9 1.0 CZ3 A:TRP123 4.3 25.9 1.0 CB A:ARG5 4.4 24.1 1.0 CE A:LYS33 4.5 29.8 1.0 CD A:LYS33 4.5 23.8 1.0 O A:HOH270 4.6 55.4 1.0 CZ A:PHE38 4.7 21.7 1.0 CG A:ARG5 4.7 25.7 1.0 NE A:ARG5 4.8 31.0 1.0 CD2 A:PHE38 4.8 21.9 1.0

K.Lim, A.Nadarajah, E.L.Forsythe, M.L.Pusey. Locations of Bromide Ions in Tetragonal Lysozyme Crystals. Acta Crystallogr.,Sect.D V. 54 899 1998.
ISSN: ISSN 0907-4449
PubMed: 9757106
DOI: 10.1107/S0907444998002844