Bromine in PDB 4a3g: Rna Polymerase II Initial Transcribing Complex with A 2NT Dna-Rna Hybrid

Enzymatic activity of Rna Polymerase II Initial Transcribing Complex with A 2NT Dna-Rna Hybrid

All present enzymatic activity of Rna Polymerase II Initial Transcribing Complex with A 2NT Dna-Rna Hybrid:
2.7.7.6;

Protein crystallography data

The structure of Rna Polymerase II Initial Transcribing Complex with A 2NT Dna-Rna Hybrid, PDB code: 4a3g was solved by A.C.M.Cheung, S.Sainsbury, P.Cramer, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

*Resolution Low / High (Å)*	49.27 / 3.50
*Space group*	C 2 2 2₁
*Cell size a, b, c (Å), α, β, γ (°)*	220.670, 394.130, 282.740, 90.00, 90.00, 90.00
*R / R_free (%)*	16.6 / 19.4

Other elements in 4a3g:

The structure of Rna Polymerase II Initial Transcribing Complex with A 2NT Dna-Rna Hybrid also contains other interesting chemical elements:

Magnesium	(Mg)	1 atom
Zinc	(Zn)	8 atoms

Bromine Binding Sites:

The binding sites of Bromine atom in the Rna Polymerase II Initial Transcribing Complex with A 2NT Dna-Rna Hybrid (pdb code 4a3g). This binding sites where shown within 5.0 Angstroms radius around Bromine atom.
In total only one binding site of Bromine was determined in the Rna Polymerase II Initial Transcribing Complex with A 2NT Dna-Rna Hybrid, PDB code: 4a3g:

Bromine binding site 1 out of 1 in 4a3g

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Bromine Binding Sites List in 4a3g

Bromine binding site 1 out of 1 in the Rna Polymerase II Initial Transcribing Complex with A 2NT Dna-Rna Hybrid

Mono view

Stereo pair view

A full contact list of Bromine with other atoms in the Br binding site number 1 of Rna Polymerase II Initial Transcribing Complex with A 2NT Dna-Rna Hybrid within 5.0Å range:

probe	atom	residue	distance (Å)	B	Occ
T:Br22 b:0.0 occ:1.00	BR	T:BRU22	0.0	0.0	1.0
	C5	T:BRU22	2.0	0.6	1.0
	C6	T:BRU22	3.0	0.1	1.0
	C4	T:BRU22	3.0	0.6	1.0
	O4	T:BRU22	3.3	0.9	1.0
	N7	T:DA21	3.3	0.4	1.0
	C5	T:DA21	3.5	0.9	1.0
	C8	T:DA21	3.6	0.5	1.0
	C4	T:DA21	3.8	0.8	1.0
	N9	T:DA21	3.9	0.7	1.0
	C6	T:DA21	4.0	0.1	1.0
	OP2	T:BRU22	4.3	0.9	1.0
	N3	T:BRU22	4.3	0.8	1.0
	C3'	T:DA21	4.3	0.1	1.0
	OE2	B:GLU1120	4.4	0.4	1.0
	N1	T:BRU22	4.4	0.7	1.0
	N6	T:DA21	4.4	0.8	1.0
	N3	T:DA21	4.6	0.1	1.0
	N1	T:DA21	4.7	0.2	1.0
	C2'	T:DA21	4.8	0.3	1.0
	OE1	B:GLU1120	4.8	0.1	1.0
	C2	T:BRU22	4.8	0.7	1.0
	C1'	T:DA21	4.9	0.6	1.0
	C5'	T:DA21	4.9	0.1	1.0
	C2	T:DA21	4.9	0.9	1.0

Reference:

A.C.Cheung, S.Sainsbury, P.Cramer. Structural Basis of Initial Rna Polymerase II Transcription. Embo J. V. 30 4755 2011.
ISSN: ESSN 1460-2075
PubMed: 22056778
DOI: 10.1038/EMBOJ.2011.396

Page generated: Wed Jul 10 20:47:26 2024

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